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Each sample must be assigned a label. For example,
post. To exclude a data file from analysis, right click on the sample and select "Remove row".
If data is paired, click
Paired and assign an unique identifier to each set.
For example, if data consists of a
post sample from each patient then the
Pair column would contain the patient's id. Each pair must contain exactly one sample from each class.
Tip: if labels are stored in Excel, copy and paste values directly into the table.
Certain analyses use florescent intensity thresholds to partition cells that express or lack a particular protein. Users can enter thresholds manually or autoassign them during analysis.
Before uploading, delete any non-analytical markers such as
CD3 that may have already been gated on before upload.
Tip: if thresholds are stored in Excel, sort both channel columns by clicking on the
Channel header and copy/paste the